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The localisation of 2‐carboxy‐ d ‐arabinitol 1‐phosphate and inhibition of Rubisco in leaves of Phaseolus vulgaris L
Author(s) -
Parry Martin A.J,
Andralojc P.J,
Lowe Helen M,
Keys Alfred J
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00038-1
Subject(s) - rubisco , phaseolus , chloroplast , photosynthesis , biochemistry , chemistry , in vivo , biology , microbiology and biotechnology , botany , gene
A recent controversial report suggests that the nocturnal inhibitor of Rubisco, 2‐carboxy‐ d ‐arabinitol 1‐phosphate (CA1P), does not bind to Rubisco in vivo and therefore that CA1P has no physiological relevance to photosynthetic regulation. It is now proved that a direct rapid assay can be used to distinguish between Rubisco‐bound and free CA1P, as postulated in the controversial report. Application of this direct assay demonstrates that CA1P is bound to Rubisco in vivo in dark‐adapted leaves. Furthermore, CA1P is shown to be in the chloroplasts of mesophyll cells. Thus, CA1P does play a physiological role in the regulation of Rubisco.