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Role of a novel photosystem II‐associated carbonic anhydrase in photosynthetic carbon assimilation in Chlamydomonas reinhardtii
Author(s) -
Park Youn-Il,
Karlsson Jan,
Rojdestvenski Igor,
Pronitalia,
Klimov Viacheslav,
Öquist Gunnar,
Samuelsson Göran
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00037-x
Subject(s) - chlamydomonas reinhardtii , thylakoid , photosynthesis , photosystem ii , chloroplast , photosystem i , carbonic anhydrase , biophysics , chlamydomonas , electron transport chain , cytochrome b6f complex , chemistry , chloroplast stroma , intracellular , biology , biochemistry , enzyme , mutant , gene
Intracellular carbonic anhydrases (CA) in aquatic photosynthetic organisms are involved in the CO 2 ‐concentrating mechanism (CCM), which helps to overcome CO 2 limitation in the environment. In the green alga Chlamydomonas reinhardtii , this CCM is initiated and maintained by the pH gradient created across the chloroplast thylakoid membranes by photosystem (PS) II‐mediated electron transport. We show here that photosynthesis is stimulated by a novel, intracellular α‐CA bound to the chloroplast thylakoids. It is associated with PSII on the lumenal side of the thylakoid membranes. We demonstrate that PSII in association with this lumenal CA operates to provide an ample flux of CO 2 for carboxylation.