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Interaction of tissue transglutaminase with nuclear transport protein importin‐α3
Author(s) -
Peng Xiaojun,
Zhang Yonghui,
Zhang Haifan,
Graner Scott,
Williams James F.,
Levitt Mark L.,
Lokshin Anna
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00018-6
Subject(s) - tissue transglutaminase , importin , nuclear transport , nuclear localization sequence , retinoic acid , nuclear protein , microbiology and biotechnology , immunoprecipitation , cell nucleus , chemistry , apoptosis , biology , biochemistry , cytoplasm , enzyme , transcription factor , gene
Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis. Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport. In this study we use the yeast two‐hybrid assay and co‐immunoprecipitation to show that tTG interacts with the nuclear transport protein importin‐α3. Using electron microscopy we demonstrate that nuclear expression of tTG in a non‐small cell lung cancer cell line is induced by retinoic acid (RA). These data suggest that importin‐α3 could mediate active nuclear transport of tTG which may be important for the regulation of critical cellular processes.