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Calcitonin gene‐related peptide decreases expression of acetylcholinesterase in mammalian myotubes
Author(s) -
Boudreau-Larivière Céline,
Jasmin Bernard J
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00015-0
Subject(s) - acetylcholinesterase , aché , calcitonin gene related peptide , medicine , endocrinology , myogenesis , calcitonin , chemistry , gene expression , western blot , microbiology and biotechnology , gene , neuropeptide , biology , skeletal muscle , enzyme , biochemistry , receptor
Nerve‐derived trophic factors are known to modulate expression of acetylcholinesterase (AChE) in skeletal muscle fibers, yet the precise identity of these factors remains elusive. In the present study, we treated mouse C2 myotubes with calcitonin gene‐related peptide (CGRP). Compared to non‐treated myotubes, cell‐associated AChE activity levels were decreased by ∼60% after 48 h of treatment. A parallel reduction in AChE total protein levels was also observed as determined by Western blot analysis. The reduction in AChE activity was due to a decrease in the levels of the G 1 molecular form and to an elimination of G 4 . By contrast, levels of secreted AChE remained unchanged following CGRP treatment. Finally, the overall decrease in AChE activity was accompanied by a reduction in AChE transcripts which could not be attributed to changes in the transcriptional rate of the ACHE gene.