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Differential expression and phosphorylation of CTCF, a c‐ myc transcriptional regulator, during differentiation of human myeloid cells
Author(s) -
Delgado M.Dolores,
Chernukhin Igor V.,
Bigas Anna,
Klenova Elena M.,
León Javier
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00013-7
Subject(s) - ctcf , phosphorylation , regulator , repressor , transcriptional regulation , myeloid leukemia , biology , regulation of gene expression , microbiology and biotechnology , myeloid , cellular differentiation , yy1 , gene , gene expression , cancer research , genetics , promoter , enhancer
CTCF is a transcriptional repressor of the c‐ myc gene. Although CTCF has been characterized in some detail, there is very little information about the regulation of CTCF activity. Therefore we investigated CTCF expression and phosphorylation during induced differentiation of human myeloid leukemia cells. We found that: (i) both CTCF mRNA and protein are down‐regulated during terminal differentiation in most cell lines tested; (ii) CTCF down‐regulation is retarded and less pronounced than that of c‐ myc ; (iii) CTCF protein is differentially phosphorylated and the phosphorylation profiles depend on the differentiation pathway. We concluded that CTCF expression and activity is controlled at transcriptional and post‐transcriptional levels.