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High polymorphism level of genomic sequences flanking insertion sites of human endogenous retroviral long terminal repeats
Author(s) -
Lavrentieva Irina,
Broude Natalia E,
Lebedev Yuri,
Gottesman Irving I,
Lukyanov Sergei A,
Smith Cassandra L,
Sverdlov Eugene D
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00004-6
Subject(s) - long terminal repeat , biology , endogenous retrovirus , genetics , genome , gene , genomic dna , retrovirus , human genome , dna , microbiology and biotechnology
The polymorphism at the multitude of loci adjacent to human endogenous retrovirus long terminal repeats (LTRs) was analyzed by a technique for whole genome differential display based on the PCR suppression effect that provides selective amplification and display of genomic sequences flanking interspersed repeated elements. This strategy is simple, target‐specific, requires a small amount of DNA and provides reproducible and highly informative data. The average frequency of polymorphism observed in the vicinity of the LTR insertion sites was found to be about 12%. The high incidence of polymorphism within the LTR flanks together with the frequent location of LTRs near genes makes the LTR loci a useful source of polymorphic markers for gene mapping.