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Cloning of a bovine orphan transporter and its short splicing variant
Author(s) -
Sakata Kazuko,
Shimada Shoichi,
Yamashita Toshihide,
Inoue Kiyoshi,
Tohyama Masaya
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01733-5
Subject(s) - complementary dna , microbiology and biotechnology , amino acid , cdna library , hek 293 cells , cloning (programming) , western blot , rna splicing , biology , northern blot , solute carrier family , peptide sequence , transporter , chemistry , biochemistry , gene , rna , computer science , programming language
We have isolated a cDNA (bv7‐3) encoding a member of the Na + ,Cl − ‐dependent transporter family and its short splicing variant (bv7‐3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7‐3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7‐3‐2. bv7‐3s contains 265 amino acids, sharing 252 N‐terminal amino acids with bv7‐3. Both mRNAs for bv7‐3 and bv7‐3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc‐tagged bv7‐3 was mainly detected on the plasma membrane, whereas myc‐tagged bv7‐3s showed a pattern of intracellular membrane staining.