Premium
Alfalfa contains substantial 9‐hydroperoxide lyase activity and a 3 Z :2 E ‐enal isomerase
Author(s) -
Noordermeer Minke A,
Veldink Gerrit A,
Vliegenthart Johannes F.G
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01706-2
Subject(s) - lyase , linoleic acid , lipoxygenase , chemistry , linolenic acid , biochemistry , isomerase , fatty acid , enzyme , stereochemistry
Fatty acid hydroperoxides formed by lipoxygenase can be cleaved by hydroperoxide lyase resulting in the formation of short‐chain aldehydes and ω‐oxo acids. Plant hydroperoxide lyases use 13‐ or 9‐hydroperoxy linoleic and linolenic acid as substrates. Alfalfa ( Medicago sativa L.) has been reported to contain a hydroperoxide lyase specific for 13‐hydroperoxy linoleic and linolenic acid only. However, in addition to 13‐hydroperoxide lyase activity we found substantial 9‐hydroperoxide lyase activity in alfalfa seedlings as well. The specific activity for 9‐hydroperoxy fatty acids was about 50% of the activity for the 13‐isomers. Furthermore, alfalfa seedlings contain a 3 Z :2 E ‐enal isomerase that converts the 3 Z ‐enal products to their 2 E ‐enal isoforms.