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Identification of a splice variant of neutrophil collagenase (MMP‐8)
Author(s) -
Hu Shou-Ih,
Klein Melissa,
Carozza Marc,
Rediske John,
Peppard Jane,
Qi Jian-Shen
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01654-8
Subject(s) - matrix metalloproteinase , splice , collagenase , identification (biology) , computational biology , chemistry , microbiology and biotechnology , medicine , biology , biochemistry , gene , enzyme , botany
We have identified a splice variant of human neutrophil collagenase (MMP‐8) transcript (MMP‐8alt) that has a 91 bp insertion between codons for amino acid residues 34 and 35 of MMP‐8 cDNA. This splice variant encodes an open reading frame for a 444 residue protein, lacking a secretory signal sequence. Our data suggested that, as opposed to the original MMP‐8, the translation product of MMP‐8alt is not a secreted protein; nevertheless, it is enzymatically active. Further studies aimed at identifying the physiological substrates of MMP‐8alt protein may lead to uncover novel roles it plays in cellular physiology.