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The aminoacceptor stem of the yeast tRNA Lys contains determinants of mitochondrial import selectivity
Author(s) -
Kazakova H.A,
Entelis N.S,
Martin R.P,
Tarassov I.A
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01653-6
Subject(s) - transfer rna , yeast , mutant , gene , mitochondrion , saccharomyces cerevisiae , biology , genetics , lysine , biochemistry , base pair , chemistry , rna , amino acid
Yeast tRNA Lys CUU is nucleus‐encoded and is partially imported into the mitochondria. Another lysine isoacceptor, tRNA Lys SUU , is also nucleus‐encoded but is not imported. These two tRNAs differ in 21 bases. We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of mutant transcripts and by expression of mutant tRNA genes in vivo we show here that the first base pair (1:72) and the discriminator base 73 are also relevant to import selectivity. Replacement of bases 1:72 in tRNA Lys SUU by those of tRNA Lys CUU makes it importable with a transport efficiency similar to natural.