Premium
RGD‐dependent growth of maize calluses and immunodetection of an integrin‐like protein
Author(s) -
Labouré Anne-Marie,
Faik Ahmed,
Mandaron Paul,
Falconet Denis
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01634-2
Subject(s) - integrin , chemistry , microbiology and biotechnology , biochemistry , biology , cell
When maize calluses are grown in the presence of the RGD peptide, important morphological changes are observed indicating the presence of a likely RGD‐binding receptor. Polyclonal antibodies generated against the human β1 integrin subunit, the platelet integrin αIIbβ3 (P23) and antibodies specific for either the β3 platelet chain or the αIIb polypeptide cross‐react with glycoproteins in Western blot analyses. Immunoprecipitation assays indicate that this maize integrin‐like protein shares structural similarities with the animal αIIbβ3 complex. We also show that AcAt2, a polyclonal antibody raised against Arabidopsis proteins purified on an RGD column, interacts with a maize protein.