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In vitro assembly of a β 2 cytochrome b 559 ‐like complex from the chemically synthesised β‐subunit encoded by the Synechocystis sp. 6803 psbF gene
Author(s) -
Francke Christof,
Loyal Rosemarie,
Ohad Itzhak,
Haehnel Wolfgang
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01629-9
Subject(s) - cytochrome , cytochrome b , protein subunit , cytochrome b6f complex , cytochrome c , cytochrome f , chemistry , stereochemistry , biochemistry , biology , photosystem i , photosystem ii , gene , enzyme , photosynthesis , mitochondrion , mitochondrial dna
The α‐ and β‐subunits of cytochrome b 559 encoded by the psbE and psbF gene, respectively, are essential components of photosystem II. The exact structure of this cytochrome is not yet known. The β‐subunit of the Synechocystis sp. 6803 cytochrome b 559 complex was synthesised by means of solid‐phase peptide synthesis. Under reducing conditions, two β‐peptide molecules could be assembled specifically with one haem to form a β 2 cytochrome b 559 ‐like complex. The spectral properties and the midpoint redox potential (48±5 mV) of the in vitro assembled β 2 cytochrome are nearly identical to those of the low potential form of the native cytochrome b 559 .