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Regulation of voltage‐dependent K + channels by methionine oxidation: effect of nitric oxide and vitamin C
Author(s) -
Ciorba Matthew A.,
Heinemann Stefan H.,
Weissbach Herbert,
Brot Nathan,
Hoshi Toshinori
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01616-0
Subject(s) - methionine , chemistry , nitric oxide , methionine sulfoxide reductase , msra , antioxidant , potassium channel , biochemistry , xenopus , oxidizing agent , biophysics , amino acid , biology , organic chemistry , gene
Methionine oxidation is known to alter functional properties of a transient A‐type potassium channel expressed in Xenopus oocytes. We show here that nitric oxide (NO) slows down the K + channel inactivation time course by oxidizing a critical methionine residue in the inactivation ball domain of the channel protein. We also demonstrate that the channel protein is protected from methionine oxidation by the enzyme methionine sulfoxide reductase and the antioxidant vitamin C.