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Production of a functional single chain antibody attached to the surface of a plant virus
Author(s) -
Smolenska Lisa,
Roberts Ian M,
Learmonth Deanne,
Porter Andrew J,
Harris William J,
Wilson T.Michael A,
Santa Cruz Simon
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01586-5
Subject(s) - potato virus x , virus , virology , conjugate , fusion protein , chemistry , antibody , biology , nicotiana benthamiana , plant virus , microbiology and biotechnology , recombinant dna , biochemistry , gene , mathematics , mathematical analysis , immunology
A potato virus X (PVX) vector was used to express a single chain antibody fragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragment. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron‐BSA conjugate. The results demonstrate that the PVX vector can be used as a presentation system for functional scFv.