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Cloning of a cDNA encoding a new calcium‐binding protein from Dictyostelium discoideum and its developmental regulation 1
Author(s) -
Han Young-Hoon,
Kang Sa-Ouk
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01568-3
Subject(s) - dictyostelium discoideum , complementary dna , cloning (programming) , dictyostelium , calcium , ef hand , microbiology and biotechnology , calcium binding protein , chemistry , biology , biochemistry , gene , computer science , organic chemistry , programming language
By employing 2D‐PAGE, a protein differentially expressed during the development of Dictyostelium discoideum was discovered. The full cDNA of this protein was cloned using RT‐PCR. The deduced protein is composed of 166 amino acid residues containing four EF‐hand domains typical for calcium‐binding proteins and was named CBP3. This protein shows little amino acid sequence homology with the other calcium‐binding proteins from D. discoideum except EF‐hand domains. The CBP3 mRNA was absent in vegetative amebas and accumulated maximally at 6 h of the development on filters. The mRNA level decreased thereafter and disappeared after 12 h of the development, while the protein level peaked at 8 h of development and remained constant thereafter. The mobility of CBP3 on SDS gel was shifted by treatment with EGTA, confirming the Ca 2+ ‐binding activity of the protein.