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Wavelength specific activation of PI 3‐kinase by UVB irradiation
Author(s) -
Kabuyama Yukihito,
Hamaya Mikiko,
Homma Yoshimi
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01565-8
Subject(s) - wortmannin , pi , intracellular , kinase , viability assay , tyrosine kinase , cell damage , microbiology and biotechnology , chemistry , biology , cell , biophysics , phosphatidylinositol , biochemistry , signal transduction
We investigated the effect of UVB irradiation on phospholipid metabolism using normal human diploid skin fibroblasts. When cells were exposed to monochromatic UV light, the intracellular PIP 3 level was increased within 1 min in a wavelength‐specific manner. The increase was most marked in the UVB range and was inhibited by a PI 3‐kinase inhibitor, tyrosine kinase inhibitor, or antioxidant reagents. Furthermore, the decrease of cell viability induced by UVB irradiation was significantly blocked by wortmannin. These results suggest that PI 3‐kinase activation is involved in novel cellular responses specific for UVB that lead to cell death.