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Functional expression and genomic structure of human chondroitin 6‐sulfotransferase 1
Author(s) -
Tsutsumi Kae,
Shimakawa Hiromi,
Kitagawa Hiroshi,
Sugahara Kazuyuki
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01532-4
Subject(s) - sulfotransferase , chondroitin sulfate , chondroitin , complementary dna , exon , biochemistry , chemistry , recombinant dna , gene isoform , microbiology and biotechnology , gene , sulfation , biology , glycosaminoglycan
The cDNA and gene encoding human chondroitin 6‐sulfotransferase (C6ST) have been cloned. The expression of a soluble recombinant form of the protein in COS‐1 cells produced an active sulfotransferase, which used as acceptor substrates polymer chondroitin, various chondroitin sulfate isoforms and chondroitin sulfate tetrasaccharides. The identification of the reaction products demonstrated that the enzyme transferred sulfate to position 6 of GalNAc in the GlcAβ1‐3GalNAc but not the IdoAα1‐3GalNAc nor the GlcAβ1‐3GalNAc(4‐ O ‐sulfate) sequences. The human C6ST gene spans more than 20 kb and consists of three exons. The protein‐coding domain of the C6ST gene is divided into two discrete exons.