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Targeted disruption of the mouse phospholipase C β3 gene results in early embryonic lethality
Author(s) -
Wang Shu,
Gebre-Medhin Samuel,
Betsholtz Christer,
Stålberg Peter,
Zhou Yinghua,
Larsson Catharina,
Weber Gunther,
Feinstein Ricardo,
Öberg Kjell,
Gobl Anders,
Skogseid Britt
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01518-x
Subject(s) - mutant , embryonic stem cell , biology , gene targeting , phenotype , embryo , microbiology and biotechnology , mutation , gene , phospholipase c , embryogenesis , genetics , signal transduction
In order to investigate the biological function of phosphatidylinositol‐specific phospholipase C (PLC) we generated mutant mice by gene targeting. Homozygous inactivation of PLCβ 3 is lethal at embryonic day 2.5. These mutants show poor embryonic organization as well as reduced numbers of cells. Identical phenotypes were recorded in homozygous mutants generated from two independently targeted embryonic stem cell clones. Heterozygous mutant mice, however, are viable and fertile for at least two generations. We also showed that mouse PLCβ 3 is expressed in unfertilized eggs, 3‐cell and egg cylinder stages of embryos. In conclusion, these results indicate that PLCβ 3 expression is essential for early mouse embryonic development.