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Increased uncoupling protein‐2 and ‐3 gene expressions in skeletal muscle of STZ‐induced diabetic rats
Author(s) -
Kageyama Haruaki,
Suga Asako,
Kashiba Misato,
Oka Jun,
Osaka Toshimasa,
Kashiwa Takayuki,
Hirano Tsutomu,
Nemoto Kiyomitsu,
Namba Yoshio,
Ricquier Daniel,
Giacobino Jean-Paul,
Inoue Shuji
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01506-3
Subject(s) - endocrinology , medicine , streptozotocin , brown adipose tissue , skeletal muscle , uncoupling protein , white adipose tissue , thermogenesis , western blot , insulin , adipose tissue , gastrocnemius muscle , diabetes mellitus , gene expression , thermogenin , biology , chemistry , gene , biochemistry
Streptozotocin (STZ)‐induced diabetic animals are vulnerable to cold stress. Uncoupling proteins (UCPs) play an important role in regulating thermogenesis. We investigated the gene expressions of UCPs in brown adipose tissue (BAT), white adipose tissue (WAT), liver and gastrocnemius muscle of STZ‐diabetic rats using Northern blot. UCP‐1, ‐2 and ‐3 mRNA expressions in BAT were all remarkably lower in STZ‐diabetic rats than those in control rats. Both UCP‐2 and ‐3 gene expressions in gastrocnemius muscle were substantially elevated in STZ‐diabetic rats and insulin treatment restored UCP gene expressions to normal levels. These results suggest that in STZ‐diabetic rats, the overexpression of UCP‐2 and UCP‐3 in skeletal muscle provides a defense against hypothermogenesis caused by decreased UCPs in BAT.