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Rapid hyaluronan uptake is associated with enhanced motility: implications for an intracellular mode of action
Author(s) -
Collis L,
Hall C,
Lange L,
Ziebell M,
Prestwich R,
Turley E.A
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01505-1
Subject(s) - motility , microbiology and biotechnology , microinjection , intracellular , cell , chemistry , cd44 , hyaluronic acid , nucleus , cell migration , biophysics , biology , biochemistry , anatomy
Texas red‐labeled hyaluronan (TR‐HA) is rapidly taken up in a CD44 independent manner into ras ‐transformed 10T1/2 fibroblasts, where it accumulates in both cell ruffles/lamellae, the perinuclear area, and the nucleus. HA does not accumulate in the cell ruffles/lamellae of parental 10T1/2 cells. Addition of HA to ras ‐transformed cells promotes their random motility but has no effect on 10T1/2 cell motility. 10T1/2 cells can be modified to take up HA into cell ruffles by exposure to phorbol ester or direct microinjection of HA into cells. Both treatments significantly stimulate 10T1/2 cell motility.