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Successful transfer of ADA gene in vitro into human peripheral blood CD34 + cells by transfecting EBV‐based episomal vectors
Author(s) -
Satoh Etsuko,
Hirai Hideyo,
Inaba Tohru,
Shimazaki Chihiro,
Nakagawa Masao,
Imanishi Jiro,
Mazda Osam
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01489-6
Subject(s) - transfection , biology , electroporation , microbiology and biotechnology , in vitro , haematopoiesis , complementary dna , virology , cd34 , adenosine deaminase , viral vector , cell culture , gene , epstein–barr virus , genetic enhancement , virus , stem cell , recombinant dna , enzyme , genetics , biochemistry
We report a novel non‐viral system for transfecting human immature hematopoietic cells in vitro. Epstein‐Barr virus (EBV)‐based episomal vectors carrying human adenosine deaminase (ADA) gene cDNA were transfected by electroporation into human peripheral blood (PB) CD34 + cells. The transgene‐specific mRNA were detected from 37 to 100% of CFU‐c (colony forming unit in culture) colonies derived from the transfected cells. A two‐fold increase in enzyme activity was also found. These results indicate the successful transfer and expression of genes in human immature hematopoietic cells using the EBV‐based episomal vector system.