Premium
Eukaryotic mRNAs encoding abundant and scarce proteins are statistically dissimilar in many structural features
Author(s) -
Kochetov Alex V,
Ischenko Igor V,
Vorobiev Denis G,
Kel Alexander E,
Babenko Vladimir N,
Kisselev Lev L,
Kolchanov Nikolay A
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01482-3
Subject(s) - messenger rna , coding region , untranslated region , translation (biology) , biology , open reading frame , translational efficiency , eukaryotic translation , context (archaeology) , polysome , microbiology and biotechnology , gene , genetics , computational biology , rna , ribosome , peptide sequence , paleontology
It is well known that non‐coding mRNA sequences are dissimilar in many structural features. For individual mRNAs correlations were found for some of these features and their translational efficiency. However, no systematic statistical analysis was undertaken to relate protein abundance and structural characteristics of mRNA encoding the given protein. We have demonstrated that structural and contextual features of eukaryotic mRNAs encoding high‐ and low‐abundant proteins differ in the 5′ untranslated regions (UTR). Statistically, 5′ UTRs of low‐expression mRNAs are longer, their guanine plus cytosine content is higher, they have a less optimal context of the translation initiation codons of the main open reading frames and contain more frequently upstream AUG than 5′ UTRs of high‐expression mRNAs. Apart from the differences in 5′ UTRs, high‐expression mRNAs contain stronger termination signals. Structural features of low‐ and high‐expression mRNAs are likely to contribute to the yield of their protein products.