Premium
A new intracellular serine protease inhibitor expressed in the rat pituitary gland complexes with granzyme B
Author(s) -
Hill Rena M,
Morresey Kate S,
Coates Leigh C,
Mezey Eva,
Fell Brennan,
Bratt Tomas,
Trapani Joseph A,
Birch Nigel P
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01475-6
Subject(s) - proteases , serpin , serine protease , granzyme , serine , recombinant dna , granzyme b , biology , microbiology and biotechnology , anterior pituitary , complementary dna , serine proteinase inhibitors , biochemistry , intracellular , granzyme a , cdna library , protease , perforin , enzyme , cytotoxic t cell , gene , in vitro , hormone
We have cloned a novel serpin (raPIT5a) from a rat pituitary cDNA library which is structurally related to members of the ovalbumin subfamily of serine protease inhibitors. This new cDNA encodes a 374‐amino acid protein, designated raPIT5a. raPIT5a was expressed in specific cells in the intermediate and anterior lobes of the pituitary. Recombinant raPIT5a was not secreted suggesting raPIT5a functions to inhibit intracellular proteases. Recombinant raPIT5a formed an SDS‐stable complex with human granzyme B, a serine protease which induces apoptosis by activating members of the caspase enzyme family. These data suggest raPIT5a may have a role in regulating granzyme B or related enzymes and apoptosis in the pituitary gland.