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Role of CFTR and anion exchanger in bicarbonate fluxes in C127 cell lines
Author(s) -
Mastrocola Teresa,
Porcelli Anna Maria,
Rugolo Michela
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01468-9
Subject(s) - intracellular ph , extracellular , cytosol , chemistry , intracellular , bicarbonate , efflux , biophysics , sodium–hydrogen antiporter , cell culture , dids , ion transporter , transfection , anion exchanger , biochemistry , microbiology and biotechnology , ion exchange , membrane , ion , biology , enzyme , sodium , genetics , organic chemistry , gene
C127 cell lines transfected with wtCFTR, ΔF508CFTR or vector were employed to determine HCO − 3 fluxes in the presence or absence of functional CFTR, using the pH‐sensitive dye BCECF. Both cytosolic alkalinization and acidification were due to activity of anion exchanger and were similar in the three cell lines, indicating that expression of CFTR did not influence anion exchanger activity. In C127wt cells only, cAMP elevating agents significantly stimulated HCO − 3 fluxes, insensitive to the inhibitor of anion exchanger 4,4′‐diisothiocyanate dihydrostilbene‐2,2′‐disulfonic acid, suggesting that activated CFTR directly mediates both HCO − 3 influx and efflux and therefore can contribute to intracellular and extracellular pH regulation.