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Molecular cloning, functional characterization and mRNA expression analysis of the murine chemokine receptor CCR6 and its specific ligand MIP‐3α 1
Author(s) -
Varona Rosa,
Zaballos Angel,
Gutiérrez Julio,
Martı́n Pilar,
Roncal Fernando,
Albar Juan Pablo,
Ardavı́n Carlos,
Márquez Gabriel
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01450-1
Subject(s) - c c chemokine receptor type 6 , microbiology and biotechnology , cc chemokine receptors , chemokine receptor , ccl20 , ccl21 , biology , c c chemokine receptor type 7 , chemokine , spleen , cd8 , receptor , chemistry , immunology , antigen , biochemistry
We have cloned the murine CCR6 receptor and its ligand, the β‐chemokine mMIP‐3α. Calcium mobilization assays performed with mCCR6 transfectants showed significant responses upon addition of mMIP‐3α. Murine MIP‐3α RNA is expressed in thymus, small intestine and colon, whereas mCCR6 RNA is expressed in spleen and lymph nodes. RT‐PCR analysis of FACS‐sorted lymphoid and antigen presenting cell subsets showed mCCR6 expression mainly in B cells, CD8 − splenic dendritic cells and CD4 + T cells. The cloning and functional characterization of the mCCR6 and mMIP‐3α will allow the study of the role of these proteins in mouse models of inflammation and immunity.