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Obtaining and characterization of EF‐hand mutants of recoverin
Author(s) -
Alekseev Andrey M,
Shulga-Morskoy Sergey V,
Zinchenko Dmitry V,
Shulga-Morskaya Svetlana A,
Suchkov Dmitry V,
Vaganova Svetlana A,
Senin Ivan I,
Zargarov Aminullah A,
Lipkin Valery M,
Akhtar Muhammad,
Philippov Pavel P
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01426-4
Subject(s) - recoverin , rhodopsin , mutant , kinase , microbiology and biotechnology , chemistry , biochemistry , biophysics , biology , gene , retinal
Several EF‐hand recoverin mutants were obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase were determined. The mutants with the ‘spoiled' 2nd, 3rd or (2nd+3rd) EF‐hand structures did not act upon the kinase activity in the μM range of Ca 2+ concentrations. Mutations of the 4th EF hand, which ‘repaired' its Ca 2+ ‐binding activity, resulted in recoverin with three ‘working' Ca 2+ ‐binding sites. The latter mutant inhibited rhodopsin kinase even more effectively than the wild‐type recoverin, containing two working Ca 2+ ‐binding structures.