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Glyoxylate cycle enzymes are present in liver peroxisomes of alloxan‐treated rats
Author(s) -
Popov Vasily N,
Volvenkin Sergei V,
Eprintsev Alexander T,
Igamberdiev Abir U
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01422-7
Subject(s) - malate synthase , glyoxylate cycle , isocitrate lyase , peroxisome , malate dehydrogenase , biochemistry , enzyme , isocitrate dehydrogenase , citrate synthase , chemistry , alloxan , biology , endocrinology , receptor , diabetes mellitus
Key enzymes of the glyoxylate cycle, isocitrate lyase (ICL) and malate synthase (MS), have been detected in the liver of alloxan‐treated rats. The activity of ICL in rat liver was 0.040 μmol/min/mg protein and the activity of MS was 0.022 μmol/min/mg protein. These enzymes were associated with the peroxisomal fraction. The activities of citrate synthase, malate synthase and malate dehydrogenase detected in the peroxisomal fraction were also increased by alloxan treatment. Isocitrate lyase was partially purified and displayed catalytic and regulatory properties similar to those of the enzyme isolated from the liver of starved rats (Popov, V.N. et al. (1996) FEBS Lett. 391, 87–90).