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The involvement of poly(ADP‐ribose) polymerase in the oxidative stress responses in plants
Author(s) -
Amor Yehudit,
Babiychuk Elena,
Inzé Dirk,
Levine Alex
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01408-2
Subject(s) - poly adp ribose polymerase , oxidative stress , programmed cell death , dna repair , dna damage , polymerase , apoptosis , microbiology and biotechnology , biology , oxidative phosphorylation , dna , chemistry , biochemistry
In plants many biotic and abiotic stresses can cause secondary oxidative stress. Earlier work showed that, depending on the severity of the oxidative stress, plants can activate either cell protective genes or programmed cell death (PCD). Poly(ADP‐ribose) polymerase (PARP) has been implicated as one of the enzymes in the apoptotic pathways induced by DNA damaging agents or oxidative stress. We show that in cultured soybean cells, PARP is involved in responses to mild and severe oxidative stresses, by mediating DNA repair and PCD processes, respectively. Addition of PARP inhibitors reduced the degree of cell death triggered by H 2 O 2 . Two windows of NAD consumption after H 2 O 2 treatment were detected. Experiments with transient overexpression of Arabidopsis PARP cDNA promoted DNA repair and inhibited cell death caused by mild oxidative stress. However, following severe stress PARP overexpression increased cell death. Expression of antisense PARP produced the opposite effects: an increase in DNA nicks and inhibition of cell death at high, but not mild doses of H 2 O 2 .