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Transcellular regulation of cell respiration by nitric oxide generated by activated macrophages
Author(s) -
Brown Guy C,
Foxwell Neale,
Moncada Salvador
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01404-5
Subject(s) - respiration , cellular respiration , nitric oxide , microbiology and biotechnology , cytochrome , cytochrome c oxidase , biochemistry , nitric oxide synthase , chemistry , cell culture , oxidase test , biology , biophysics , enzyme , anatomy , genetics , organic chemistry
A macrophage cell line (J774), activated with interferon‐γ and endotoxin to express the inducible form of NO synthase (iNOS), immediately inhibited the cellular respiration of co‐incubated L‐929 fibroblasts or non‐activated J774 macrophages. The inhibition was potent, rapid and reversible when the NO was removed by adding oxyhaemoglobin or by inhibiting iNOS. Exogenously added NO also rapidly and reversibly inhibited cellular respiration over the same range of NO concentrations. This inhibition was competitive with oxygen and due to direct inhibition of cytochrome oxidase. Thus, NO generated by one cell can regulate the respiration of adjacent cells, supporting the hypothesis that NO may be a physiological and/or pathological regulator of cellular respiration, via its inhibition of cytochrome oxidase.