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Creatine supplementation improves intracellular Ca 2+ handling and survival in mdx skeletal muscle cells
Author(s) -
Pulido S.M,
Passaquin A.C,
Leijendekker W.J,
Challet C,
Wallimann T,
Rüegg U.T
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01399-4
Subject(s) - phosphocreatine , creatine , duchenne muscular dystrophy , medicine , endocrinology , skeletal muscle , endoplasmic reticulum , myogenesis , creatine kinase , extracellular , chemistry , myocyte , cytosol , intracellular , muscular dystrophy , biology , biochemistry , enzyme , energy metabolism
Dystrophic skeletal muscle cells from Duchenne muscular dystrophy (DMD) patients and mdx mice exhibit elevated cytosolic Ca 2+ concentrations ([Ca 2+ ] c ). Pretreatment of mdx myotubes for 6–12 days with creatine (20 mM) decreased the elevation in [Ca 2+ ] c induced by either high extracellular Ca 2+ concentrations or hypo‐osmotic stress to control levels. 45 Ca 2+ influx measurements suggest that creatine lowered [Ca 2+ ] c by stimulating sarcoplasmic reticulum Ca 2+ ‐ATPase. Creatine pretreatment increased levels of phosphocreatine but not ATP. Furthermore, myotube formation and survival were significantly enhanced by creatine pretreatment. Therefore, creatine supplementation may be useful for treatment of DMD.