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The effect of Lp3 enlargement on the folding and catalysis of hepatitis delta virus cis ‐cleaving ribozyme
Author(s) -
Wu Huey-Nan,
Lee Jing-Yu,
Ping Yueh-Hsin,
Wang Chen-Nai
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01395-7
Subject(s) - ribozyme , mammalian cpeb3 ribozyme , hairpin ribozyme , oligonucleotide , mutant , vs ribozyme , chemistry , biology , microbiology and biotechnology , rna , biochemistry , gene
Oligonucleotides were inserted into the Lp3 region of a hepatitis delta virus (HDV) cis ‐cleaving ribozyme and the effect of Lp3 enlargement on ribozyme folding was examined by assaying the activity of each mutant. The location of insertion and the sequence of the inserted oligonucleotide had distinct effects on ribozyme activity, and the HDV ribozyme was capable of adopting the active structure for cis ‐cleavage when a pentanucleotide was inserted into the 3′ portion of Lp3. Furthermore, whether the insertion mutant cis ‐cleaved or not, the structure of Lp3 was altered by the inserted oligonucleotide. These findings disclose that the 5′ rather than the 3′ portion of Lp3 is critical for ribozyme folding and catalysis.