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Cytochrome c release from brain mitochondria is independent of the mitochondrial permeability transition
Author(s) -
Andreyev Alexander Y.,
Fahy Brenda,
Fiskum Gary
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01394-5
Subject(s) - egta , mitochondrion , mitochondrial permeability transition pore , cytochrome c , biophysics , cytochrome , swelling , permeability (electromagnetism) , chemistry , inner mitochondrial membrane , mitochondrial apoptosis induced channel , biochemistry , membrane potential , membrane , calcium , biology , apoptosis , programmed cell death , enzyme , materials science , organic chemistry , composite material
Ca 2+ uptake by brain mitochondria induces the release of up to 40% of total cytochrome c in a cyclosporin A‐insensitive manner. In the presence of ATP and Mg 2+ , this process is not accompanied by mitochondrial swelling. There is a moderate decrease in membrane potential under these conditions, but it is completely reversible upon removal of accumulated Ca 2+ by addition of EGTA+A23187 but not by EGTA alone. These observations provide evidence that cytochrome c release from brain mitochondria does not require the membrane permeability transition. However, brain mitochondria can undergo the permeability transition in the absence of ATP and Mg 2+ , which results in cyclosporin A‐sensitive large amplitude swelling, loss of Ca 2+ uptake capacity and release of matrix solutes.