Premium
Molecular cloning and functional expression of the human glycine transporter GlyT2 and chromosomal localisation of the gene in the human genome 1
Author(s) -
Morrow J.A.,
Collie I.T.,
Dunbar D.R.,
Walker G.B.,
Shahid M.,
Hill D.R.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01390-8
Subject(s) - complementary dna , glycine receptor , microbiology and biotechnology , biology , neurotransmitter transporter , open reading frame , gene , glycine , sarcosine , amino acid , molecular cloning , cloning (programming) , human genome , radiation hybrid mapping , genetics , biochemistry , transporter , chromosome , gene mapping , peptide sequence , genome , computer science , programming language
Neurotransmitter transport systems are major targets for therapeutic alterations in synaptic function. We have cloned and sequenced a cDNA encoding the human type 2 glycine transporter GlyT2 from human brain and spinal cord. An open reading frame of 2391 nucleotides encodes a 797 amino acid protein that transports glycine in a Na + /Cl − ‐dependent manner. When stably expressed in CHO cells, human GlyT2 displays a dose‐dependent uptake of glycine with an apparent K m of 108 μM. This uptake is not affected by sarcosine at concentrations up to 1 mM. Radiation hybrid analysis mapped the GlyT2 gene to D11S1308 (LOD=8.988) on human chromosome 11p15.1–15.2.