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Effects of cold acclimation and palmitate on energy coupling in duckling skeletal muscle mitochondria
Author(s) -
Roussel Damien,
Rouanet Jean-Louis,
Duchamp Claude,
Barré Hervé
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01382-9
Subject(s) - respiration , thermogenesis , acclimatization , mitochondrion , skeletal muscle , chemistry , biology , biochemistry , medicine , endocrinology , anatomy , adipose tissue , botany
Gastrocnemius subsarcolemmal and intermyofibrillar mitochondria were isolated from 5‐week‐old cold‐acclimated and thermoneutral control ducklings. In vitro respiration (polarography) and ATP synthesis (bioluminescence) were determined at 25°C. Subsarcolemmal mitochondria showed a higher state 4 respiration and lower respiratory control and ADP/O ratio in cold‐acclimated than in thermoneutral ducklings. Palmitate decreased the rate of ATP synthesis in both mitochondrial populations to about 30% of maximal but failed to abolish this process even at high concentrations. It is suggested that both expensive ATP synthesis and increased ATP hydrolysis could contribute synergistically to muscle non‐shivering thermogenesis in cold‐acclimated ducklings.