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DNA sequence recognition by bis‐linked netropsin and distamycin derivatives
Author(s) -
Grokhovsky S.L,
Surovaya A.N,
Burckhardt G,
Pismensky V.F,
Chernov B.K,
Zimmer Ch,
Gursky G.V
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01379-9
Subject(s) - netropsin , stereochemistry , footprinting , dna , chemistry , sequence (biology) , base pair , nucleotide , biochemistry , base sequence , gene , minor groove
We studied the interaction of cis ‐diammine Pt(II)‐bridged bis‐netropsin, cis ‐diammine Pt(II)‐bridged bis‐distamycin and oligomethylene‐bridged bis‐netropsin with synthetic DNA fragments containing pseudosymmetrical AT‐rich nucleotide sequences and compared it with the interaction of the parent compounds netropsin and distamycin A. For fragments containing multiple blocks of (A/T) 4 and (T/A) 4 separated by zero, one, two and three GC‐base pairs, DNase I footprinting and CD spectroscopy studies reveal that 5′‐TTTTAAAA‐3′ is the strongest affinity binding site for cis ‐diammine Pt(II)‐bridged bis‐netropsin and bis‐distamycin. They both bind less strongly to a DNA region containing the sequence 5′‐AAAATTTT‐3′. Netropsin, distamycin A and oligomethylene‐bridged bis‐netropsin exhibit far less sequence discrimination.