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Human D 3 dopamine receptor in the medulloblastoma TE671 cell line: cross‐talk between D 1 and D 3 receptors
Author(s) -
Levavi-Sivan Berta,
Park Bae-Hang,
Fuchs Sara,
Fishburn C.Simone
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01356-8
Subject(s) - agonist , receptor , downregulation and upregulation , biology , dopamine , endocrinology , cell culture , stimulation , dopamine receptor , medicine , microbiology and biotechnology , chemistry , biochemistry , genetics , gene
In search of a cell line in which the D 3 dopamine receptor is expressed endogenously, we found that the neuron‐derived human medulloblastoma cell line TE671 expresses the human D 3 (hD 3 ) and D 1 (hD 1 ) receptor, but neither the D 2 or D 4 receptors. Exposure of TE671 cells to the D 3 agonist 7‐OH‐DPAT (DPAT), or to the D 1 agonist SKF‐38393 (SKF) increased the expression of hD 3 or hD 1 mRNA, respectively. Moreover, whereas DPAT had no effect on hD 1 mRNA levels, stimulating the cells with SKF caused an increase in both hD 1 and hD 3 transcript levels. These results suggest (i) that following ligand stimulation, hD 3 and hD 1 receptors are upregulated to enhance their own receptor expression, and (ii) that upregulation of hD 1 receptor transcripts leads to a stimulation of the hD 3 dopamine receptor transcripts.