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Activation of muscarinic K + current by β‐adrenergic receptors in cultured atrial myocytes transfected with β 1 subunit of heterotrimeric G proteins
Author(s) -
Bender Kirsten,
Wellner-Kienitz Marie-Cécile,
Meyer Thomas,
Pott Lutz
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01350-7
Subject(s) - heterotrimeric g protein , muscarinic acetylcholine receptor , pertussis toxin , g protein , muscarinic acetylcholine receptor m2 , gi alpha subunit , receptor , protein subunit , gq alpha subunit , chemistry , transfection , myocyte , g protein coupled inwardly rectifying potassium channel , carbachol , medicine , isoprenaline , endocrinology , biology , stimulation , biochemistry , gene
Muscarinic K + channels (I K(ACh) ) in native atrial myocytes are activated by βγ subunits of pertussis toxin (Ptx)‐sensitive heterotrimeric G proteins coupled to different receptors. βγ subunits of Ptx‐insensitive G s , coupled to β‐adrenergic receptors, do not activate native I K(ACh) . In atrial myocytes from adult rats transfected with rat brain β 1 subunit I K(ACh) can be activated by stimulation of β‐adrenergic receptors using isoprenaline. This effect is insensitive to Ptx. These findings demonstrate for the first time promiscuous (Ptx‐insensitive) coupling of G sβγ to GIRK channels in their native environment.