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Kinetic and spectroscopic characterization of an intermediate peroxynitrite complex in the nitrogen monoxide induced oxidation of oxyhemoglobin
Author(s) -
Herold Susanna
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01345-3
Subject(s) - peroxynitrite , chemistry , methemoglobin , peroxynitrous acid , nitrogen , photochemistry , inorganic chemistry , kinetics , carbon monoxide , nitrite , reactive nitrogen species , nitric oxide , hemoglobin , catalysis , organic chemistry , enzyme , nitrate , superoxide , physics , quantum mechanics
Stopped‐flow spectroscopy studies of the nitrogen monoxide mediated oxidation of oxyhemoglobin in the pH range 5–10.5 show that an intermediate can be characterized at alkaline pH. The rate of decay of this species to methemoglobin increases significantly with decreasing pH such that it does not accumulate in quantities large enough to be observed under neutral or acidic conditions. Kinetic and spectroscopic properties of this intermediate support its assignment as a methemoglobin peroxynitrite complex.