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Transformed but not normal hepatocytes express UCP2
Author(s) -
Carretero M.Victoria,
Torres Luis,
Latasa M.Ujue,
Garcı́a-Trevijano Elena R,
Prieto Jesús,
Mato José M,
Avila Matı́as A
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01335-0
Subject(s) - northern blot , complementary dna , microbiology and biotechnology , clone (java method) , biology , western blot , cell culture , messenger rna , parenchyma , southern blot , gene expression , cell , gene , biochemistry , genetics , botany
Uncoupling protein 2 (UCP2) expression in liver is restricted to non‐parenchymal cells. By means of differential display screening between normal rat liver and H4IIE hepatoma cells we have isolated a cDNA clone encompassing part of UCP2 cDNA. Northern blot analysis revealed that UCP2 is expressed in some hepatocarcinoma cell lines, while it is absent in adult hepatocytes. UCP2 mRNA in H4IIE cells was downregulated when cells were cultured for 36 h in 0.1% serum and its expression was restored upon addition of 10% serum or phorbol esters. Hypomethylation of UCP2 was observed in transformed UCP2 expressing cells. Our results indicate that UCP2 is expressed in some hepatocarcinoma cell lines and that serum components may participate in maintaining elevated UCP2 levels.