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Cloning and functional characterisation of the mouse P2X 7 receptor
Author(s) -
Chessell I.P,
Simon J,
Hibell A.D,
Michel A.D,
Barnard E.A,
Humphrey P.P.A
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01332-5
Subject(s) - cloning (programming) , receptor , microbiology and biotechnology , chemistry , computational biology , biology , genetics , computer science , programming language
We have isolated a 1785‐bp complementary DNA (cDNA) encoding the murine P2X 7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80% and 85% homology to the human and rat P2X 7 subunits, respectively. Functional properties of the heterologously expressed murine P2X 7 homomeric receptor broadly resembled those of the P2X 7 receptor in the native cell line. However, marked phenotypic differences were observed between the mouse receptor, and the other P2X 7 receptor orthologues isolated with respect to agonist and antagonist potencies, and the kinetics of formation of the large aqueous pore.