Premium
Molecular analysis of regulation of gene expression of the human erythroid anion exchanger (AE) 1
Author(s) -
Philipp Stephan,
Kneussel Matthias,
König Jörg,
Appelhans Heribert
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01326-x
Subject(s) - band 3 , gene , microbiology and biotechnology , transcription (linguistics) , promoter , gene expression , anion exchanger , biology , transcription factor , regulation of gene expression , chemistry , membrane protein , genetics , ion exchange , membrane , ion , linguistics , philosophy , organic chemistry
The anion exchange protein AE1 is the most abundant membrane protein in human erythrocytes mediating the electroneutral chloride/bicarbonate exchange. We identified a promoter region in the 5′ flanking region of the human AE1 gene which controls transcription in a cell type independent manner. In addition a second, distal promoter element mediates gene expression only in erythroid cells and in dependence upon differentiation. Within this distal promoter region we defined a 44 bp sequence containing a novel CT‐rich motif with very strong promoter activity whereas a second 28 bp segment suppresses gene expression.