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Complex formation of the nonstructural protein 3 of hepatitis C virus with the p53 tumor suppressor
Author(s) -
Ishido Satoshi,
Hotta Hak
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01312-x
Subject(s) - ns3 , immunoprecipitation , chemistry , suppressor , amino acid , c terminus , microbiology and biotechnology , hepatitis c virus , virus , virology , biochemistry , biology , gene
By co‐immunoprecipitation analysis, we demonstrated that wt‐p53 formed a complex with non‐structural protein (NS) 3 of hepatitis C virus, both in the absence and the presence of NS4A, a viral cofactor that strongly associates with NS3. Deletional analysis revealed that a portion near the N‐terminus of NS3 (amino acids (aa) 1055 and 1200), which is different from the NS4A binding site, was necessary for the complex formation with wt‐p53. On the other hand, a portion near the C‐terminus of wt‐p53 (aa 301–360), which has been reported to contain the oligomerization domain, was important for the complex formation with NS3.