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F 420 H 2 :NADP oxidoreductase from Methanobacterium thermoautotrophicum : identification of the encoding gene via functional overexpression in Escherichia coli
Author(s) -
Berk Holger,
Thauer Rudolf K.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01288-5
Subject(s) - escherichia coli , methanobacterium , oxidoreductase , enzyme , archaea , gene , bacteria , biology , biochemistry , microbiology and biotechnology , halophile , chemistry , genetics
F 420 H 2 :NADP oxidoreductase is found in methanogenic, sulfate‐reducing and halophilic archaea and also in some bacteria. The putative gene encoding the enzyme was cloned from Methanobacterium thermoautotrophicum (strain Marburg) and heterologously expressed in Escherichia coli . The overproduced active enzyme was purified, characterized and crystallized.