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Characterisation of a novel Drosophila melanogaster testis specific PP1 inhibitor related to mammalian inhibitor‐2: identification of the site of interaction with PP1
Author(s) -
Helps Nicholas R.,
Vergidou Cornillia,
Gaskell Terri,
Cohen Patricia T.W.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01286-1
Subject(s) - drosophila melanogaster , protein phosphatase 1 , biology , microbiology and biotechnology , melanogaster , phosphatase , biochemistry , yeast , saccharomyces cerevisiae , peptide sequence , gene , enzyme
A novel Drosophila melanogaster protein, termed inhibitor‐t, that bears 41% sequence similarity to human protein phosphatase inhibitor‐2 has been identified using human protein phosphatase 1 (PP1) in the yeast two hybrid system. Inhibitor‐t mRNA is detected in adult males, larvae and pupae and the 184 amino acid thermostable protein located only in testis. The gene for inhibitor‐t maps to cytological location 86F1 on the third chromosome. Bacterially expressed inhibitor‐t specifically inhibits both mammalian and D. melanogaster PP1 catalytic subunits with an IC 50 of approximately 200 nM. A motif ‐FEX 1 X 2 RK‐, conserved between inhibitor‐t, inhibitor‐2 and its Saccharomyces cerevisiae homologue Glc8, is demonstrated to be required for binding to PP1.