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Enhancement of the immunogenicity of a synthetic peptide bearing a VP3 epitope of hepatitis A virus
Author(s) -
Pintó Rosa M,
González-Dankaart Juan F,
Sánchez Gloria,
Guix Susana,
Gómara M.José,
Garcı́a Mónica,
Haro Isabel,
Bosch Albert
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01278-2
Subject(s) - immunogenicity , epitope , virology , hepatitis a virus , peptide , hepatitis c virus , bearing (navigation) , virus , chemistry , computational biology , biology , biochemistry , antibody , immunology , computer science , artificial intelligence
The immune responses elicited in mice by different forms of the VP3(110–121) B‐epitope of the hepatitis A virus (HAV) were studied. Different forms of incorporation in liposomes were tested, encapsulation, rather than surface exposure, being the best antigenic preparation. Three larger peptides of the VP3 epitope, two of them containing a hepatitis B virus T‐epitope, and a third containing a putative T‐epitope of HAV (VP3(102–121)) were assayed. While this latter T‐epitope induced an enhancement of the response against the VP3 B‐epitope, the artificially coupled T‐epitopes failed to induce a significant increase. The administration of two multiple antigenic peptide (MAP) constructs, the first containing the VP3(110–121) and VP1(11–25) HAV sequences and the second only the VP1(11–25) sequence, also suggested the presence of a T‐epitope, since the response against the VP1 peptide was increased in the first construct.