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Transgenic Cre recombinase expression in germ cells and early embryogenesis directs homogeneous and ubiquitous deletion of loxP‐flanked gene segments
Author(s) -
Bergqvist Ingela,
Eriksson Björn,
Eriksson Maria,
Holmberg Dan
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01272-1
Subject(s) - cre recombinase , biology , transgene , germline , genetically modified mouse , gene , transgenesis , recombinase , cre lox recombination , microbiology and biotechnology , gene expression , genetics , embryogenesis , oogenesis , recombination
We report on the establishment of a transgenic mouse line expressing Cre recombinase under control of the c‐kit promoter. Expression of Cre recombinase was only observed in late spermatogenesis and oogenesis, however, Cre‐mediated deletion of floxed gene segments occurred at this stage as well as in early embryogenesis. As a consequence of this, a chimeric distribution of loxed alleles was found in a large fraction of these mice. The chimerism was very homogeneous in different organs and tissues of the same individual but varied between different individual offspring. The potential uses for this mouse line are discussed.