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Synthetic lipid products of PI3‐kinase which are added to culture medium prevent low K + ‐induced apoptosis of cerebellar granule neurons via Akt kinase activation
Author(s) -
Shimoke Koji,
Yamada Masashi,
Ikeuchi Toshihiko,
Hatanaka Hiroshi
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01235-6
Subject(s) - protein kinase b , phosphatidylinositol , apoptosis , kinase , cerebellum , granule (geology) , microbiology and biotechnology , chemistry , programmed cell death , biochemistry , biology , neuroscience , paleontology
To examine which lipid product of phosphatidylinositol 3‐kinase (PI3‐K) is essential for the survival‐promoting pathway in cultured cerebellar granule neurons, three synthetic derivatives of lipid products of PI3‐K were added to culture medium containing a low concentration (5 mM) of potassium (LK + ) which induces apoptotic cell death. We found that dipalmitoylphosphatidylinositol 3,4‐bisphosphate and dipalmitoylphosphatidylinositol 3,4,5‐trisphosphate, but not dipalmitoylphosphatidylinositol 3‐monophosphate, effectively blocked the LK + ‐induced apoptosis. These two synthetic phospholipids increased Akt activity but not that of PI3‐K. These findings demonstrated that specific lipid products of PI3‐K which are added to culture medium activate Akt/PKB without modulating PI3‐K itself, and as a result prevent neuronal cell death in cerebellar granule neurons.