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UV‐C radiation induces apoptotic‐like changes in Arabidopsis thaliana
Author(s) -
Da Antoine,
Gallois Patrick
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01208-3
Subject(s) - dna fragmentation , apoptosis , fragmentation (computing) , programmed cell death , arabidopsis thaliana , protoplast , biology , dna damage , dna , microbiology and biotechnology , nuclear dna , plant cell , arabidopsis , biophysics , chemistry , botany , biochemistry , mitochondrial dna , mutant , gene , ecology
With a view to studying programmed cell death in plants at the molecular level, we report here for the first time that apoptotic‐like changes are induced by UV radiation in plant nuclei. In Arabidopsis thaliana seedlings a UV‐C dose of 10–50 kJ/m 2 induces an oligonucleosomal DNA fragmentation which is reminiscent of the apoptotic DNA ladder described in animal cells. This DNA fragmentation was also detected in situ in protoplast nuclei as soon as 2 h after UV‐C treatment. Moreover, UV‐C induced a nuclear morphology characteristic of animal apoptotic nuclei. We propose that UV‐C induction constitutes a powerful tool to compare the cellular response to irreversible UV damage in plants to that in animals and to study programmed cell death in A. thaliana .