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The regulated expression of an intrabody produces a mutant phenotype in Drosophila
Author(s) -
Hassanzadeh Gh Gholamreza,
Devoogdt Nick,
Ghysen Alain,
De Baetselier Patrick,
Muyldermans Serge,
Dambly-Chaudière Christine
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01205-8
Subject(s) - phenocopy , mutant , biology , phenotype , gene , transgene , drosophila (subgenus) , genetics , gene expression , green fluorescent protein , microbiology and biotechnology , gene targeting
Intrabodies show great promise for controlling gene expression. As an initial attempt to evaluate the intrabody technology in Drosophila , the gene poxn was used as target. Transgenic flies harboring different anti‐Poxn scFv genes integrated into various chromosomes were obtained. In one transformant, a phenocopy resembling the hypomorphic poxn − phenotype was produced in embryos and larvae following induction of expression of α‐Poxn2 intrabody. The antisense approach was used as control. Parameters that can affect the success of intrabody technology are described.