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Identification of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two‐hybrid analysis and functional complementation tests of yeast mutants
Author(s) -
Mizoguchi Tsuyoshi,
Ichimura Kazuya,
Irie Kenji,
Morris Peter,
Giraudat Jérôme,
Matsumoto Kunihiro,
Shinozaki Kazuo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01197-1
Subject(s) - complementation , arabidopsis , mutant , arabidopsis thaliana , map kinase kinase kinase , yeast , biology , protein fragment complementation assay , bimolecular fluorescence complementation , saccharomyces cerevisiae , mitogen activated protein kinase kinase , microbiology and biotechnology , mapk cascade , mapk/erk pathway , protein kinase a , kinase , genetics , gene
A possible MAP kinase (MAPK) cascade of Arabidopsis thaliana was identified on the basis of both yeast 2‐hybrid analysis and complementation analysis of yeast mutants. Specific protein‐protein interactions between ATMPK4 (a MAPK) and MEK1 (a MAPKK) and interactions between MEK1 and ATMEKK1 (a MAPKKK) were detected by using the 2‐hybrid system. A growth defect of the yeast mpk1 Δ mutant was reversed by coexpression of ATMPK4 and MEK1. Coexpression of the N‐terminal deletion form of ATMEKK1 increased the ability of MEK1 to suppress a growth defect of the yeast pbs2 Δ mutant. These results suggest that ATMPK4, MEK1, and ATMEKK1 may interact with each other and constitute a specific MAPK cascade in Arabidopsis . This is the first demonstration of a possible MAPK cascade in plants.